Arl8b Knockout cell line (BHK-21)

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location： Home > Products > KO Cell Lines > Arl8b Knockout cell line (BHK-21)

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Arl8b Knockout cell line (BHK-21) Order

Arl8b Knockout cell line (BHK-21)
Catalog#
YKO-R069
Size
1*10^6
Storage conditions
Shipped on dry ice; Store in liquid nitrogen
Arl8b Knockout cell line (BHK-21)
Order

Detailed Product Information

Product category
KO Cell Lines
cell name
Arl8b Knockout cell line (BHK-21)
Morphology
Epithelial-like, adherent
Passage ratio
1：2-1：3
Species
Gene Name
Arl8b
Gene ID
101836258
Gene Information
NCBI
Construction method
Electroporation/Lentivirus
Mycoplasma test
negative
Culture method
90%DMEM+10%FBS
Target region
Knockout region: Exon 2 (based on transcript Arl8b-201)
Validation: PCR+Sanger Sequencing
Arl8b Knockout cell line (BHK-21) Target region

Instruction

Use exclusive Red Cotton system to design a sgRNA fragment knockout strategy for the Arl8b gene, clone the two sgRNAs into CRISPR-U™ vector, transfect the vector into the BHK-21 cell line by electroporation/lentivirus method, use the limited dilution method for single-cell cloning and use the EZ-editor™ Monoclone Genotype Validation Kit (Cat# YK-MV-1000) for nucleic acid lysis and PCR amplification on the single-cell clones, followed by Sanger sequencing to verify the genotype. Upon passing the verification, Arl8b gene knockout Arl8b Knockout cell line (BHK-21) will be expanded and cryopreserved.
Knockout Cell Line Work Flow and Validatio Knockout Cell Line Work Flow and Validatio

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